摘要
应用放射免疫法测定正常发情配种的14头奶牛外周血浆中LH浓度的变化。在输精同时肌注LRH-A_3,于注射后2.5小时出现一个较高的L H分泌峰,峰高达39.40±15.94ng/ml,而注射生理盐水的对照组LH仍维持基础水平,0.70～1.2ng/ml。对垂体的兴奋试验表明,在不同的生理状况下动物对LRH-A_3的反应性不同,发情期高于黄体期。通过对奶汁中孕酮含量的测定,发现肌注LRH-A_3后1～6天内孕酮含量达1.57ng/ml,显著高于对照组0.47ng/ml(P<0.01);连续测定27天,肌注LRH-A_3的怀孕牛平均孕酮含量为10.30±3.76ng/ml,高于未孕牛及其他各组(P<O.05)。在授精的同时肌注LRH-A_3,可以促进卵泡进一步成熟和排卵,提高性细胞的受精率,促进黄体形成并提高其分泌功能,减少早期胚胎死亡,从而提高受胎率。
The luteinizjng hormone ( LH ) concentrations were determinated by radioimmunoassay in the peripheral blood plasma from 14 dairy cows with normal estrus and copulation, the results proved that administration of LRH-A 3 by intramuscular injection at the same time of insemination c-ould promote follicles to maturation and ovulati-on, raise luteal secretion function and reduce eia-rly embyonal death, thus the pregnancy rate was increased.
出处
《中国兽医科技》
CSCD
1992年第2期10-13,共4页
Chinese Journal of Veterinary Science and Technology
关键词
放射免疫
测定
奶牛
受胎率
radioimmunoassay, LRH-A3, blackwhite dairy cow, pregnancy rate
