摘要
目的 探讨多西紫杉醇 (docetaxel,TXT)对小鼠血管肉瘤细胞株的增殖抑制作用。方法 采用 Alamar Blue法检测 TXT和其他 5种抗肿瘤药对体外培养 ISOS- 1细胞的增殖抑制作用 ;复制鼠血管肉瘤模型 ,2 0只移植瘤模型小鼠分为 4组 ,每组 5只 ,即 1 5 mg/kg TXT组 ;2 1 0mg/kg TXT组 ;3 2 0 mg/kg TXT组 ;4对照组。静脉给予 TXT治疗 ,每周 1次。结果 体外试验 ,TXT对 ISOS- 1的 IC50 为 1 5.8ng/ml,比其他 5种抗肿瘤药物明显低 ;体内试验 ,TXT三个剂量组瘤体积抑制率均达 70 %以上 ,与对照组比较有显著性差异 (P<0 .0 1 ) ,且经 TXT治疗的荷瘤小鼠生存期优于对照组。结论 TXT对恶性度高的鼠血管肉瘤细胞株 ,体内、体外试验具有明确的增殖抑制作用及延长生存期的效果 ,是治疗血管肉瘤有应用前景的抗肿瘤药物。
Objective To study the proliferation inhibition effect of docetaxel (TXT) on murine angiosarcoma cell line (ISOS-1). Methods To determine the proliferation inhibition effect of TXT and other five kinds of anti-tumor drugs on ISOS-1 cells in vitro by Alamar Blue assay. Animal model of murine-phenotypic angiosarcoma was established and twenty mouse models were divided averagely into four groups: treated group with TXT of 5mg.kg-1, treated group with TXT of 10mg.kg-1, treated group with TXT of 20mg.kg-1 and control group. TXT was administrated by mainline once a week. Results In the study in vitro, the IC50 of TXT, which was obviously lower than that of the other 5 kinds of chemical drugs, was 15.8 ng.ml-1. In the in vivo study, all the inhibitory rates of tumor volume were more than 70% in TXT groups, and the survival days of mice were longer than those of the control. Conclusions TXT had obvious effects of proliferation inhibition and prolongation of survival days on high grade malignant angiosarcoma in the in vivo and vitro study. TXT should be a very useful anti-tumor drug in the treatment of angiosarcoma in the future.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2004年第3期241-242,共2页
Chinese Journal of Gerontology
