摘要
目的 探讨丙型肝炎病毒 (HCV) 1b型患者HVR1 ,NS5A区准种复杂性与干扰素疗效的关系。方法 应用单链构象多态性聚合酶链反应 (SSCP)法检测慢性丙型肝炎患者干扰素治疗前后HCV准种的动态变化。结果 治疗前HCVHVR1准种的SSCP条带数 (复杂性 )≤ 3者在经干扰素治疗后HCVRNA阴转率为 71 .4 3% ,>3者HCVRNA阴转率为 1 5 .38% ,两者相比 ,差异有显著性 (P <0 .0 5 ) ;治疗有效组SSCP条带数为 2 .4 3± 0 .98( x±s) ,无效组为 4 .4 6± 0 .88,两者相比 ,差异亦具显著性 (P <0 .0 1 )。而NS5A区SSCP条带数则无差异。结论 HCV1b型慢性丙型肝炎患者HVR1区准种的复杂性与干扰素疗效有关 ;而NS5A区准种的复杂性与干扰素疗效无关。
Objective To investigate the relationship of HCV HVR1 and NS5A quasispecies complexity and the response to interferon (IFN) therapy in patients with HCV 1b infection. Methods Dynamic changes of HCV quasispecies before and after IFN therapy were detected using the method of polymerase chain reaction mediated single strand conformation polymorphism (PCR SSCP). Results After patients reveived IFN treatment, HCV RNA negative transformation rates in SSCP bands of HCV HVR1 ≤3 and >3 (detected prior to IFN treatment) were 71.43% and 15.38% , respectively, there was significant difference between them. The number of SSCP bands was 2.43 ± 0.98 (±s) and 4.46 ± 0.88 (±s) in effective patients group and ineffective patients group respectively, there was also significant difference between them, but there was no difference in SSCP bands of NS5A quasispecies. Conclusion The complexity of HCV HVR1 quasispecies was related with the curative effect of IFN therapy in patients with chronic hepatitis C and there was no correlation between the complexity of NS5A quasispecies and the curative effect of IFN therapy.
出处
《中国感染控制杂志》
CAS
2004年第2期103-105,共3页
Chinese Journal of Infection Control
