摘要
建立了肾复康胶囊中野黄芩苷和芦丁的反相高效液相色谱测定方法。以甲醇 水为提取溶剂,采用超声提取法对样品进行前处理。以0 02mol/L磷酸二氢钾缓冲溶液(含1 0%(体积分数)冰醋酸) 甲醇(体积比为63∶37)为流动相,于330nm波长下检测,肾复康提取液中野黄芩苷和芦丁可达基线分离,分析时间在20min内。野黄芩苷和芦丁在10～300mg/L内,其峰面积与浓度之间线性关系良好,目标物的加标回收率大于98%。该方法适用于肾复康胶囊及相关药材中野黄芩苷和芦丁黄酮类化合物的测定和质量控制。
A rapid and simple method for the determination of scutellarin and rutin in Shenfukang capsules has been developed using reversed-phase high performance liquid chromatography. Scutellarin and rutin were first extracted by ultrasonic extraction with methanol-water ((1∶1, v/v)). The resulting extracts were separated on a C_(18) column (4.6 mm i.d.×150 mm, 5 μm). The mobile phase consisting of 0.02 (mol/L) KH_2PO_4 solution containing 1.0% glacial acetic acid-methanol (63∶37, v/v) was run at a flow rate of 1.0 (mL/min). The detection wavelength was 330 nm and the injection volume was 20 μL. Good linearity in the range of 10 mg/L to 300 (mg/L) was obtained for scutellarin and rutin. The method was successfully used for the quantification of scutellarin and rutin of Shenfukang capsules.
出处
《色谱》
CAS
CSCD
北大核心
2004年第2期138-140,共3页
Chinese Journal of Chromatography
