摘要
为进一步明确对肿瘤细胞发生直接作用的 LAK 样活性细胞属何淋巴细胞亚群,本实验取5例胃癌患者胃周淋巴结,分离出淋巴细胞,在体外以 rIL—2诱导培养出有杀伤肿瘤靶细胞活性的 LAK 样活性细胞,用免疫组织化学染色方法,对 LAK 样活性细胞与胃癌细胞共同孵育2hr 时的细胞涂片,进行了细胞亚群分析.同时对 LAK 样活性细胞同胃癌靶细胞作用2hr 时的状态进行原位包埋固定,制成电镜标本,直观地证实了与胃癌靶细胞粘附的 LAK 样活性细胞的表型特征及亚群属性,并在电镜下展示了 LAN 样活性细胞与胃靶细胞作用2hr 时的形态.本实验结果表明,粘附在胃癌细胞周围的 LAK 样活性细胞中,CD8^+者所占百分比远比其在总的 LAK样活性细胞的高;几乎所有粘附在胃癌靶细胞周围的 LAK 样活性细胞都是 TAC^+淋巴细胞.
Two hour after LAK—like cells were co—cultured with gastric cancer cells,the mixed sus-
pension was spread on slids and dyed with histochemical method.Through analysing the dyed
cells,we directly confirmed what subset the LAK—like cells belonged to.The featrue of LAK—
like cells adhering to cancer cell was observed under electron micrscope in our experiment.A-
mong the lymphoctes which were adherent to cancer cells,the number of CD8^+ cells was in-
creased.Most of adherent to cancer cells,the number of CD8^+ cells was increased.Most of ad-
herent cells were TAC^+,suggesting that the expression of TAC antigen plays an important role
in cell mediated cytotoxity.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1992年第6期361-364,共4页
Chinese Journal of Immunology
关键词
LAK细胞
免疫组织化学
胃肿瘤
癌
gastric neoplasm
LAK cell
Immunohistochemistry
lymphocytic subset
