摘要
采用两种识别不同抗原决定簇的抗尿激酶(UK)单克隆抗体建立了测定人 UK 含量的夹心法ELISA.本法灵敏度为0.15ng/ml,批内 CV4.3%,批间 CV8.7%,平均回收率98%.应用本法测定了部分正常细胞和肿瘤细胞株培养上清中的 UK 含量,肿瘤细胞培养上清 UK 量明显高于正常细胞.测定82名正常人血浆中 UK 含量,其结果为1.31±0.6ng/ml.
A sandwich ELISA for the measurement of urokinase(UK)antigen was developed based on anti—UK monoclonal antibodies(McAbs)against two non—overlapping epitopes.The lower limit of sensitivity of the assay was 0.15ng/ml.Coefficients of variation of the assay at physilogi-cal levels of UK were 4.3 percent within assaysw and 8.7 percent between assays.The recovery of added UK was about 98 percent.Culture media of some human malignant cell lines contains more UK than that of normal cell lines measured by our assay.The ELISA was used to measure
the concentration of UK in plasma from 82 healthy donors.The mean value for the healthy donors was 1.31±60.6ng/ml of UK in plasma.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1992年第5期308-310,共3页
Chinese Journal of Immunology
