摘要
将一带有人白细胞的介素2cDNA 的大肠杆菌——酵母菌穿酸质粒转化啤酒酵母Saccharomyces cerevisiae 8534-8c,再将转化子与另一啤酒酵母 Saccharomyces cervisiae CSH83—L 进行杂交,得到二倍体菌株.同时,还研究了单倍体转化子和二倍体菌株在表达重组白细胞介素2上的差异以及不同培养条件下二倍体菌株的表达情况,确定了合适的发酵条件,并进行了五立升自动发酵罐的发酵研究.
We transformed a E.coli—yeast shuttle plasmie containing human interleukin 2 gene into a yeast strain Saccharomyces cerevisiae 8534—8c,then hybredized the transformants with another yeast strain Saccharomyces cerevisiae CSH 83—L to get diploid hy brids The hap Joid transfor-mants and diploid gybreds were compared for their expression level of recombinant interleukin 2.We also studied the gybrids'rIL—2 expression under various conditions and its fermentation.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1992年第5期272-275,共4页
Chinese Journal of Immunology
关键词
白细胞介素2
啤酒酵母
重组
转化
Human interleukin 2
Yeast
Recombinant
Transformantion
Hybridization
Expression
Fermentation
