摘要
应用单克隆抗体结合 APAAP 酶标记法,对6～11周的人早期胚肝中淋巴细胞相关的分化抗原和组织相容性抗原 HLA—DP 的表达进行研究的结果表明:在6～11周的胚肝中没有 CD7和 CD2抗原阳性细胞存在,提示早期胚肝中可能不存在 T 淋巴细胞的原位发生。B 淋巴细胞相关抗原的表达情况有所不同,其中 CD19、CD20、CD22显示为阴性;而 CD9、CD10、CD38抗原自6周胚肝中即有表达,表明这三种抗原的表达早于胚肝中前 B 细胞的出现.观察中发现 CD9^+、CD10^+和 CD38^+细胞呈现多种不同大小和形态,这一结果提示这三类抗原可能是存在于多种造血祖细胞上的共同抗原。组织相容性抗原 HLA—DP 在6～11周的胚肝中也表现为阳性。对胚肝冰冻切片的标记研究显示 CD9^+、CD10^+、CD38^+和 HIA—DP^+细胞呈随机散在分布,8或10周之后分别有不同程度的增殖细胞群存在。
The McAb and APAAP method were used in the studies on the expression of some lym-phocyte associated antigens(CDs)and HLA—DP in the human fetal liver of 6—11 wks,emby roniclage,It is showed that to CD7^+,or CD2^+ cells was detected in fetal liver of 6—11 wks,indi-cating that probably no in-suit generating of T lymphocyte occured in human early fetal liver.The expression of some B lymphocyte associated antigens was found to be varied.No CD19^+,CD20^+ or CD22^+ cells was observed.As contrast,The CD9^+ CD10^+ and CD38^+ cells were found with increased percentage during 6 to 11 wks in fetal liver,this suggest that these three antigens appeared before the presence of pre—B cells in fetal liver.CD9,CD10 and CD38 antigens,being found existing in cells which varied in size and shape,probably are the common antigens shared by progenitors of more than one lineages of hemapoietic cells in fetal liver.The histocompatibility antigen HLA—DP is also positive during 6—11 wks.In frozen scetions,the CD9 ^+,CD^+,CD38^+and HLA—DP^+ cells were scatted singly over the fetal liver in random.But after 8 or 10 wks of
gestational age,some positiwe cells aggregations were found,probably indicating the prolifera—tion of these cells.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1992年第4期213-218,共6页
Chinese Journal of Immunology
关键词
类早期胚肝
淋巴细胞
分化抗原
Human fetal liver
iymphocyte
Differentiated antigen
