摘要
目的 :建立用反相高效液相色谱 (RP HPLC)测定枳实药材中橙皮苷含量的方法。方法 :采用色谱柱为HypersilODS2 5μmC1 8柱 (2 50mm× 4 6mm) ;流动相为乙腈 1 %冰醋酸水溶液 (2 0∶80 ) ;检测波长 :2 83nm ;参比波长 360nm ;流速 1 0mL/min。结果 :橙皮苷的线性范围分别为 0 40 7～ 3 2 56μg,橙皮苷的平均回收率为 1 0 1 5 %。结论 :RP HPLC测定枳实药材中橙皮苷含量的方法 ,不仅操作简便、准确 。
Objective: To establish the determining the quantity of hesperidin in Fructus Aurantii immaturus. Method: Hesperidin were separated on a Hypersil ODS2 C18 column with acetonitrile-1% glacial acetic acid (the volume ratio of 20:80) as the mobile phase and detected at the wavelength of 283 nm with the reference wavelength of 360 nm. The flow rate was 1.0 mL/min . Result: The calibration curves were linear in the ranges of 0.407～3.256 μg for hesperidin ,the average rate of recovery were 101.5% for hesperidin . Conclusion: Determining the quantity of hesperidin in Frutus aurantii immaturus by RP-HPLC is simple ,accurate and recurrent.
出处
《江西中医学院学报》
2004年第2期62-63,共2页
Journal of Jiangxi College of Traditional Chinese Medicine
