摘要
目的 建立一种简便、灵敏、特异的DNA检测方法。方法 用荧光偏振技术检测 10 2例乙型肝炎患者血清中的HBVDNA ,并与多聚酶链反应 酶联免疫吸附试验 (PCR ELISA)方法作比较。结果 该方法可以检测出 1× 10 1拷贝的HBVDNA模板 ,CV为 6 .4 4 % ,对 10 2例乙型肝炎患者的血清进行检测 ,HBsAg( + )、HBeAg( + )、抗HBc( + )血清HBVDNA阳性率为 10 0 % ( 4 0 / 4 0 ) ;HBsAg( + )、抗HBe( + )、抗HBc( + )血清HBVDNA阳性率为 81.8% ( 2 7/ 33) ;HBsAg( + )、抗HBc( + )血清HBVDNA阳性率为 72 .4 % ( 2 1/ 2 9) ,其余为阴性。 30例非乙型肝炎患者血清中HBVDNA的检测结果均为阴性。结论 该方法简单、灵敏、特异 。
Objective To establish a simple, sensitive and specific method for detecting HBV DNA in serum. Methods Hundred two serum samples from patients with hepatitis B were detected by PCR fluorescence polarization (PCR FP) and results obtained were compared with PCR ELISA. Results 1×10 1 copy HBV DNA could be detected by the method( CV =6.44%), positive rates for Serum samples with HBsAg (+), HBeAg (+), anti HBc (+) were 100%(40/40); Serum samples with HBsAg (+), anti HBe (+), anti HBc (+) were 81.8% (27/33); Serum samples with HBsAg (+), anti HBc (+) were 72.4%(21/29). Thirty normal sera were all negative for HBV DNA. Conclusions PCR FP is a simple, rapid, sensitive and specific method, it can be used for detecting pathogen DNA in clinical samples.
出处
《检验医学》
CAS
北大核心
2004年第2期128-130,共3页
Laboratory Medicine
