摘要
以H5N1亚型禽流感病毒接种鸡胚 ,收取鸡胚液为抗原研制了禽流感灭活疫苗 ,并对制苗抗原以及疫苗的物理性状、安全性、免疫效力、免疫剂量和保护期等进行了试验。结果表明 ,获得了较高效价的制苗抗原 ,灭活前后HA分别为 2 10 0～ 11 0 和 2 9 0～ 10 0 。试制疫苗物理性状符合要求 ,安全性良好。试验鸡在免疫 14d后血清抗体达到2 7 5,2 10d血清HI抗体仍维持在 2 5 0 左右 ;免疫后 18d使用高致病性H5亚型禽流感病毒攻毒 ,获得 10 0 %的保护。分别使用 0 1,0 3和 0 5mL免疫鸡 ,发现 0 3～ 0 5mL剂量组免疫后血清抗体达到 2 7 4～ 8 0 。疫苗在 2～ 8℃保存5 4 0d ,疫苗物理性状没有改变 ,免疫后血清HI抗体可以达到 2 7 5。
The H5N 1 strain of avian influenza virus (AIV) was inoculated into chicken embryo, collected the dead embryo, after which it was inactivated and made into oil adjuvant vaccine. The antigen, physical characteristic,, safety, efficacy, dosage and protection period were tested according to the requirement of new biological product. Results showed the high quality antigen was harvested, the HA of the antigen was up to 2 10.0-11.0 and 2 9.0-10.0 respectively during the actived and inactived period. The inactived vaccine was safe; its physical characterization was also fit for the requirement of the oil-adjuvant vaccine. 14 day later, the HI antibody rose to 2 7.5 and maintained 2 5.0 on day 210. The all vaccined poultry acquired protection challenged with AIV-H 5N 1 strain after 18-day vaccination. If the birds were injected with 0.1, 0.2 and 0.3 mL respectively, the HI antibody rose to 2 7.4-8.0 in latter two groups. No obvious physical change was observed for 540 days when the vaccine was conserved between 2 and 8℃,and the HI antibody responsed to AIV-H 5 was 2 7.5 . The above results suggest that the vaccine can be used to preventthe epidemic AIV-H 5N 1.
出处
《中国农业大学学报》
CAS
CSCD
北大核心
2004年第1期41-44,共4页
Journal of China Agricultural University
