摘要
目的 :检测MHCⅡ类分子反式激活因子Ⅰ型启动子 (CIITA pI)是否具有启动活性及其在树突状细胞 (dendriticcell,DC)中的特异启动活性。方法 :利用荧光素酶报告系统 ,检测仅 112bp的Ⅰ型CIITA pI在DC中的特异启动活性及报告基因在DC不同成熟程度下的表达。结果 :该启动子在DC中能够特异启动报告基因的表达 ,而在非DC细胞中几乎无启动活性 ;其启动活性随DC的成熟程度而上升。结论 :为进一步应用CIITA
AIM: To detect whether CIITA-pI can drive reporter gene expression and its specific promoter activity in DCs. METHODS: Luciferase reporter system was used to detect 112 bp CIITA-pI-specific promoter activity in DCs. RESULTS: CⅡTA-pI could drive expression of reporter gene in DCs but not in HEK 293 cells and its promoter activity increased with the maturation of DCs. CONCLUSION: Our work provides a basis for further study of targeting DC gene therapy using CⅡTA-pI as DC-specific promoter.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2004年第1期55-57,共3页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金资助项目 (No .39970 2 70 )
