摘要
目的 建立高效液相色谱法测定保胃丸中芍药苷的含量。方法 采用ODS色谱柱(4.6mm×250mm),以甲醇-异丙醇-36%乙酸-水(25∶2∶2∶71)为流动相;检测波长为230nm。结果 芍药苷的含量线性范围为0.4～2.0μg,r=0.9997,平均回收率为97.86%,RSD为1.99%。结论 该方法简便、准确。
Objective To develop a HPLC method for determining Paeoniflorin in Baowei Pill. Method ODS column(4.6mm×250mm) was used with methanol-iso Propanol-36% acetic acid-water (25∶2∶2∶71) as mobile phase. Detection wavelength was 230 nm. Result Paeoniflorin was separated well. Good linearity of Paeoniflorin was obtained (r=0.9997) within the range of 0.4～2.0μg. The average recovery was 97.86% and RSD was 1.99%. Conclusion This method was simple and accuracy.
出处
《中国中医药信息杂志》
CAS
CSCD
2004年第3期222-223,共2页
Chinese Journal of Information on Traditional Chinese Medicine
