摘要
目的 探讨 RT- PCR检测胃癌区域淋巴结微转移及其意义。方法 以病理组织学和 RT- PCR扩增角蛋白 1 9(K1 9)对 1 8例胃癌患者的癌组织标本及 78个淋巴结进行检测。结果 1 8例胃癌癌组织均有 K1 9m RNA表达 ,78个淋巴结中 ,组织学阳性 1 6个 ,而 RT- PCR阳性达 2 6个。结论 RT- PCR扩增 K1 9m RNA检测胃癌区域淋巴结微转移是一种敏感、特异的检测方法 ,结合淋巴结微转移的情况 ,对胃癌患者手术淋巴结清扫范围有一定的参考价值 ,有助于更精确的分期及对预后的判断。
Objective To explore the clinic significance of reverse transcription polymerase chain reaction (RT-PCR) in detecting gastric cancer micrometastases in regional lymph node.Methods Tumor tissues of 18 cases and 78 lymph nodes were studied by both histopathologic examination and RT-PCR. Results All specimens of 18 gastric carcinoma expressed keratin 19 (k19) mRNA, and there were 16 positive lymph node metastases in examined lymph nodes by routine histological examination, the number reach to 26 when the specincens were examined by RT-PCR. Conclusions K19 mRNA detection by RT-PCR is a high sensitive and specific method, combining micrometastases, it is helpful to decide extent of lymph node dissection and determine the tumor stage and predict prognosis precisely.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2004年第4期296-297,共2页
Chinese Journal of Gerontology
