摘要
根据GenBank中登录的传染性腔上囊病病毒(IBDV)标准强毒株52 70株基因组序列,设计并合成了1对扩增IBDVVP2基因的特异性引物。以IBDV超强毒分离株HN01感染发病鸡腔上囊组织中提取的病毒RNA为模板,用RT PCR扩增出了1.45kb的cDNA产物,将扩增的IB DVHN01株VP2基因克隆于pMD18 T载体上,获得了pMD18 T VP2重组质粒。将IBDVHN01株VP2基因序列测定结果与已发表的其他IBDV毒株VP2基因序列进行分析比较,绘出系统进化树。结果表明,HN01株与欧洲超强毒株UK661、日本超强毒株OKYM、香港超强毒株HK46等非常相似,而与经典强毒株、弱毒株和变异株相差较大。
According to the published sequence of the standard strain 52-70 of infectious bursal (disease) virus (IBDV), a pair of primers was designed and synthesized to amplify the very virulent IBDV HN01 strain's VP2 gene. The VP2 gene's cDNA fragment 1.45 kb in length was obtained with the primers by RT-PCR. Then the fragment was cloned into pMD 18-T vector at SmaⅠ site and was sequenced by the Sanger's DNA sequencing method. The corresponding amino acid sequence was deduced from sequence of the fragment mentioned above. The nucleotide sequence was compared with the published sequences of VP2 gene of five strains respectively, and the deduced amino acid sequence was also compared with the published amino acid sequences from VP2 gene of the five strains respectively. Results showed that HN01 strain is close to the very virulent strain UK661 and OKYM, but is different from the other IBDV strains.
出处
《中国兽医科技》
CSCD
北大核心
2004年第4期62-66,共5页
Chinese Journal of Veterinary Science and Technology
基金
国家"863"计划项目(2002AA245051)
