摘要
目的 :构建小鼠白细胞介素 1 8(m IL - 1 8)的重组真核表达质粒 ,制备有效的免疫佐剂。方法 :通过RT- PCR分别从 BAL B/c小鼠和 Swiss小鼠肝组织总 RNA中扩增 m IL - 1 8,并将其连接于载体 pc DNA3中 ,再用双酶切及测序方法鉴定重组质粒。结果 :构建出两组重组质粒 ,其中从 BAL B/c小鼠中获得的 m IL - 1 8序列与Genbank中序列完全一致 ,而从 Swiss小鼠中获得的 m IL - 1 8序列有两个点突变。结论 :重组质粒 pc IL - 1 8构建成功 ,且发现 m IL - 1 8具有未见报道的点突变 ,已登录于 Genbank。这为研制抗肿瘤 DNA疫苗 。
ObjectiveTo construct recombinant Murine IL 18 (mIL 18) plasmid which could be expressed in eukaryotic systems and be used as immunoadjuvant. MethodsmIL 18 was amplified from BALB/c and Swiss mouse liver mRNA by RT PCR, and ligated orientatively into the eukaryotic expressing plasmid pcDNA3. The recombinant plasmids, pcIL 18,were evaluated with endorebonuclease digestion and sequencing. ResultsTwo recombinant plasmids were constructed. The sequence of mIL 18 from BALB/c mouse was consistent with that of Genbank, while the one from Swiss mouse had two point mutations. ConclusionTwo recombinant plasmids pcIL 18 are constructed successfully. They will play a key role in reinforcing DNA vaccine. And gene polymophism in mIL 18 has been found and received by Genbank (AY362457).
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2004年第2期206-208,共3页
Journal of Jilin University：Medicine Edition
基金
卫生部临床学科重点科研项目 (2 0 0 1 31 4 5 )
吉林省科技厅重点科研项目 (2 0 0 2 0 4 0 3)
吉林省卫生厅科研项目(0 2 1 )
吉林大学医疗新技术新疗法基金项目 (2 0 0 1 )
吉林大学创新基金 (2 0 0 2 )
