摘要
目的 探讨苯丙酸诺龙(NP)对烧伤♂大鼠靶器官睾丸及性激素水平的影响。方法 建立20％体表面积深Ⅱ度烫伤大鼠动物模型,治疗组伤后第2天始,隔日一次im NP 5 mg·kg-1,对照组以生理盐水代替,每隔10天分别断头处死治疗组及对照组各4只大鼠。监测两组大鼠血中性激素水平变化及观察睾丸组织病理切片和电镜照片的变化。结果 两组睾酮、雌二醇、间质细胞刺激素、促卵泡素质量浓度在各时间点无显著性差异(P>0.05)。在10、20 d两个时间点,两组血睾酮的均值小于30 d后创面愈合后各组的均值,有显著性差异(P>0.05),其余时间点前后对照无显著性差异。而雌二醇、促黄体生成素、促卵泡素浓度前后对照无明显规律。光镜下睾丸组织切片两组间无明显结构差异。治疗后10 d电镜下仅见对照组精原细胞及初级精母细胞内超微结构有轻度损伤现象,而治疗组未见异常。治疗后30、60 d两组细胞内超微结构均正常,无明显差异。结论 在烧伤病理条件下,应用治疗剂量的NP60 d对♂大鼠的睾丸结构无明显损害作用,对性激素水平无明显影响。
OBJECTIVE To evaluate the effects of Nandrolone phenylpropionate (NP) on sex hormone, testis in major burned male rats. METHODS Adult male SD rats with a deep second- degree cutaneous burned,20% total body surface area were randomly divided into two groups to receive either 5 mg·kg-1 NP(NP group) or normal saline as placebo (control group) every other day.Levels of testrone (T) ,estradiol (E2) ,luteinizing hormone(LH) and follicle stimulating hormone(FSH) were measured on the post - burn days of 10,20,30,40,50 and 60. Tissue section of testis were observed by light microscope and electron microscope. RESULTS The levels of T, E2, FSH, LH and the structure and ultrastructure of testis between NP group and control group had no significant difference at every time - point. The side influence of NP on testis seemed very limited within this dosage and the treatment period.CONCLUSION Nandrolone phenylpropionate (NP) had no significant effects on the levels of sex hormone, and had no significant lesion on testis tissues.
出处
《华西药学杂志》
CAS
CSCD
2004年第2期100-102,共3页
West China Journal of Pharmaceutical Sciences
