摘要
本文对分离自云南的森林脑炎病毒进行了动物敏感性研究,实验证明云南森林脑炎病毒对小白鼠有较强的致病性,三日龄乳鼠无论经脑内、腹腔、皮下接种均能致病、死亡,但毒力较国内森林脑炎病毒标准株低;三周龄小白鼠经鼻腔接种亦能发病致死。对乳大白鼠、幼年豚鼠和金黄色地鼠能引起发病或死亡,病毒抗原定位主要在脑组织。病理检查表明感染的各种动物脑组织均有明显病变。此外,对鸡胚敏感,能引起BHK_(21)、Vero、Vero-E_6等传代细胞及人胚肾、乳猪肾原代细胞的CPE_0结果表明了云南森林脑炎病毒对细胞、动物的致病性与国内森林脑炎病毒标准株相似,仅毒力稍低。
In 1989, 3 strains of Yunnan RSSE virus were isolated from 2 groups of Ixodes Ovatus (T56, T57) and blood of a female patient with fever of unknown infected with YHV, T56v, T57v by intracerebral, intraperitoneal, nasal cavity and subcutaneous inoculation respectively.The incubation period is 3—4 days. The 3 strains of virus could multiply and caused death of chicken embryo, baby rat, young guinea-pig, golden hamster, tupaia belangeri. After infection, the animals showed anorexia, weak, thin, ruffled fur and paralysis. The virus antigen have been demostrated in the brain tissue of animals by IFAT. The pathological changes of the brain tissues of the infected animals are hyperaemia, neuron deterioration and necrosis. The 3 strains of virus could readly multiply and induce CPE in BHK-21, Vero, Vero-E6, SPK, HEK, MEF cells
出处
《中国病毒学》
CSCD
1992年第4期383-388,共6页
Virologica Sinica
关键词
病毒
森林脑炎
敏感性试验
Russian spring-summer encephalitis virus SenSitization test
