摘要
背景:高脂血症的长期存在可促进动脉粥样硬化(atherosclerosis,AS)的形成和发展,在此过程中生长因子所起的作用越来越受重视,但对血管内皮生长因子(vascular endothelial growth factor, VEGF)的作用还不十分清楚。 目的:探讨VEGF在AS形成过程中的作用。 设计:随机对照的实验研究。 地点、材料和干预:本实验在沈阳体育学院研究生实验室完成。实验动物为C57BL/6J(易感AS型)纯系小黑鼠20只。实验动物被随机分两组:对照组10只,脂肪乳组10只。对照组喂以常备标准饲料;脂肪乳组除正常喂养外,每日以脂肪乳灌喂方式按10mL/kg进行高脂膳食。 主要观察指标:高脂血症与非高脂血症小鼠主动脉组织光镜下形态学改变、主动脉壁的VEGF蛋白表达、血脂水平比较。 结果:对照组主动脉壁VEGF蛋白表达均为阴性,仅外膜残存脂质VEGF阳性。脂肪乳组血栓中、局部增生的新内膜均有VEGF蛋白表达。血脂测定结果:脂肪乳组的总胆固醇、三酰甘油、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,HDL-C)分别为(4.97±0.10),(2.24±0.26),(2.93±0.17)mmol/L较对照组[分别为(4.17±0.29),(1.22±0.10),(2.44±0.33)mmol/L]明显升高(t=2.364,2.335,2.939,P<0.01);脂肪乳组HDL-C[(1.29±0.24)mmol/L]较对照组[(1.50±0.16)mmol/L]
BACKGROUND:Long-term hyperlipidemia can promote atherogenesis and increasing attention is being drawn to the effect of various growth factors in this process.However,the effect of vascular endothelial growth factor (VEGF) remains unclear.
OBJECTIVE:To study the effect of VEGF on atherogenesis.DESIGN:A randomly controlled trial study.
SETTING,PARTICIPANTS and INTERVENTION:Ten female and ten male C57BL/6J mice of 8 weeks old were randomly divided into 2 groups:normal control group(NC) and atherogenic diet group(AS) with 10 mice in each.The mice in NC group were fed standard feed,while those in AS were given lipid milk prepared by us 10 mL/kg every day apart from the normal feed.MAIN OUTCOME MEASURES:Lipids(TC,TG,HDL,LDL) in serum were measured;the expression of VEGF protein in aortic wall was detected by immunohistochemical SABC method.
RESULTS:(1)In AS group the level of TC,TG,LDL increased (t = 2.364,2.335,and 2.939,respectively,P < 0.01),but the level of HDL decreased (t = 2.231,P < 0.05) compared with that of NC group.(2)In NC group,there was no expression of VEGF protein in aortic wall and the expression of VEGF protein only in the remaining lipid of adventitia was positive.In AS group,the expression of VEGF protein was found in thrombus and proliferating neonate tunica ultima.
CONCLUSION:In the condition of hyperlipidemia,endogenetic VEGF participates in thrombosis and proliferation of tunica intima and promotes the occurrence and development of atherosclerosis.
出处
《中国临床康复》
CSCD
2004年第9期1766-1767,F003,共3页
Chinese Journal of Clinical Rehabilitation
