摘要
目的 :探讨用不同方法检测淋巴瘤标本中EB病毒LMP 1的表达率以选择一种敏感、可靠的检测手段。方法 :运用SP法、CSA法检测EB病毒LMP 1在Raji细胞株细胞学涂片、12 3例淋巴瘤 (其中 10 4例NHL ,19例HL)切片中的表达 ,部分病例结合PCR加以验证。结果 :(1)细胞学涂片SP阳性细胞率为 70±10 % ,CSA法 10 0 %阳性。 (2 )HL中 ,SP法检测LMP 1阳性率为 6 / 19(32 % ) ,CSA法阳性率 8/ 19(4 2 % ) ,PCR阳性率为 11/ 19(5 8% )。 (3)非霍奇金淋巴瘤 (NHL)中SP法阳性率为 3/ 10 4 (其中B NHL 1/ 79,1 2 6 % ,T NHL2 / 2 5 ,8% )。CSA法阳性率为 9/ 10 4 ,其中B NHL4 / 79(5 % ) ,T NHL 5 / 2 5 (2 0 % ) ,ITCL达 2 / 10 (2 0 % )。结论 :SP法在检测低丰度抗原时漏检率较高 ,CSA法为一种较敏感、可靠的检测手段 ,结合PCR方法可有效检测低丰度抗原。
Objective:To choose a sensitive and reliable way of examination through using different methods to check the performance rate of LMP-1 in the lymphoma cases.Methods:using SP method,CSA method to examine the performance of LMP-1 on the Raji cell smear and the slides of 123 lymphoma cases (104/123 are NHL,19/123 are HL).Some cases used the PCR method too.Results:(1)the cell rate of SP Positive on the cytology smear is 70±10%;(2)Among HL,LMP-1 Positive rates examined by SP method is 6/19(32%),by CSA method is 8/19(42%),by PCR method is 11/19(58%);(3)Among NHL,Positive rate examined by SP method is 3/104(B-NHL 1/79,1.26%;T-NHL 2/25,8%);by CSA method 9/104(B-NHL 4/79,5%;T-NHL 5/25,20%,ITCL 2/10,20%).Conclusion:SP method has a higher mission in checking low-density antigen,whiole,the CSA method is a more sensitive and reliable method,which can check them effectively with the help of the PCR method.
出处
《上海生物医学工程》
2004年第1期3-6,共4页
Shanghai Journal of Biomedical Engineering
