摘要
目的:探讨CD4^+ T细胞在2、4、6-三硝基苯磺酸(TNBS)诱导的大鼠结肠炎发病过程中的变化。方法:采用免疫组化、流式细胞术和Western-blot方法观察TNBS诱导的结肠炎大鼠CD4^+ T细胞的数目及其蛋白表达的变化。结果:TNBS诱导的结肠炎大鼠外周血和肠黏膜CD4^+ T细胞数目增加,肠系膜淋巴结CD4^+ T细胞蛋白表达升高。结论:TNBS诱导的大鼠结肠炎发病过程中CD4^+T细胞明显增加,与人类结肠炎具有相似之处,本模型为研究结肠炎治疗药物提供了新的作用靶点。
AIM: To study on the expansion of CD^(4+) T lymphocytes isolated from rats with colitis induced by 2,4,6-trinitrobenzene sulphonic acid (TNBS). METHODS: The numeration and protein expression of CD^(4+) T lymphocytes had been performed by immunohistological analysis, flow cytometry and Western-blot. RESULTS: The numeration of CD4+ T cells and the protein expression of CD^(4+) T lymphocytes increased significantly in rats with colitis induced by 2,4,6-trinitrobenzene sulphonic acid. CONCLUSION: Expansion of CD^(4+) T lymphocytes in TNBS-induced colitis provides us a simple and reducible animal model for research of the new target of therapeutic drug.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2004年第3期347-350,共4页
Chinese Journal of Pathophysiology
基金
国家自然科学基金(No.30100329)
