摘要
目的 :体外定向诱导大鼠骨髓基质干细胞 (BMSCs)向神经元样细胞的分化。方法 :用DMEM培养液冲洗骨髓 ,收集骨髓细胞接种在培养瓶中体外扩增、纯化。用诱导剂诱导BMSCs分化为神经元样细胞。用免疫组化ABC法鉴定神经丝蛋白 (NF 2 0 0 )、微管相关蛋白 (MAP 2 )、神经元特异核蛋白 (NeuN)、巢蛋白 (Nestin)、胶质纤维酸性蛋白 (GFAP)、GAD和ChAT的表达。结果 :BMSCs经诱导后 ,胞体增大 ,并伸出细长突起 ,与神经元形态相似。免疫组化显示大部分细胞NF 2 0 0 ,MAP 2 ,NeuN和Nestin表达阳性 ,(3± 0 .8) %的细胞GAD表达阳性 ,(5± 0 .3) %的细胞ChAT表达阳性 ,而GFAP表达阴性。结论 :BMSCs可被诱导分化为神经元样细胞 ,部分细胞可表达GAD与ChAT。
Objective To investigate the differentiation of the rat bone marrow stromal cells (BMSCs) into neuron like cells which express glutamic acid decarboxylase (GAD) and choline acetyltransferase (ChAT). Methods BMSCs of rats were flushed out with DMEM from femurs marrow and separated, and then planted in plastic flask. After being expanded and purified,the BMSCs were differentiated into neuron-like cells. Neurofilament (NF-200), microassociate protein-2 (MAP-2), neuron-specific nuclear protein (NeuN), nestin, gial fibrillary acidic protein (GFAP), GAD and ChAT were detected by immunohisitochemistry. Results The induced BMSCs exhibited neuronal morphological characteristics,and the expressions of NF-200, MAP-2, NeuN and nestin were positive, but the expression of GFAP was negative. About(3±0.8)% of the induced BMSCs expressed GAD, and (5±0.3)% expressed ChAT. Conclusion BMSCs can differentiate into neuron like cells, and express GAD and ChAT partially.
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2004年第1期18-20,共3页
Journal of Central South University ：Medical Science
基金
国家自然科学基金 (3 0 0 70 2 5 3 )
