摘要
本研究是通过细胞化学方法在植物抗寒锻炼中揭示环腺苷酸磷酸二酯酶(cAMP-PDEase)活性的变化。在抗寒锻炼前,适宜温度生长的麦苗细胞内,cAMP-PDEase活性主要定位分布于质膜及核仁和染色质上;当低温抗寒锻炼时,强抗寒性小麦(Triticum aestivum L.)品种麦苗细胞内的cAMP-PDEase活性显著受到抑制,脱锻炼后又恢复;弱抗寒性品种的酶活性则变化较小。这些结果说明,抗寒锻炼中cAMP-PDEase活性的改变与植物抗寒锻炼中抗寒力的提高有密切关系。讨论了该酶对抗寒锻炼调控作用的途径。
Comparative studies on localization and distribution of cyclic adenosine monophosphate phosphodiesterase (cAMP-PDEase) activity in the young leaf cells of two different cold resistant wheat (Triticum aestivum L.) varieties during cold acclimation were carried out by means of ultracytochemical method. The results indicated that the reaction products of cAMP-PDEase activity in two varieties of the seedlings grown at optimum temperature (20—25℃) were mainly localized at plasmalemma,. nucleoli and chromatin. When the wheat seedlings were subjected to low temperature acclimation, cAMP-PDEase activity in the strong cold resistant variety Yanda 1817 seedlings was markedly decreased; however, Iittle alteration of this enzyme activity was observed in the weak cold resistant variety Zhengzhou 39-1. cAMP-PDEase ac- tivity was recovered after deacclimation. The results suggested that changes in cAMP-PDEase activity during cold acclimation were closely related to the development of plant cold hardiness.
基金
国家自然科学基金资助项目
关键词
环腺苷酸磷酸
二酯酶
小麦
越冬性
Cyclic adenosine monophosphate phosphodiesterase
Plant cold hardiness
Wheat
