摘要
延胡索(Corydalis yanhusuo)培养物的生长和生物碱的积累动态表明:生物碱的积累大体上随着培养物的增殖而逐渐增加;某些生物碱如延胡索乙素等,在培养物生长进入静止期后仍然继续积累。蜜环菌(Armillaria mellea)液作为诱导子能促进某些生物碱的积累,如原鸦片碱的含量通过蜜环菌液的诱导可达到干重的0.114%,而对照组中只有痕迹量。
It has shown that alkaloid accumulation in tissue cultures of Corydalis yanhusuo orchestrated to some extent the growth in cultures. Some alkaloids, such as tetrahydropalmatine continued to increase in the static phase of culture growth. The culture solution of Armillaria mellea as an eliciror was able to promote alkaloids accumulation in cultures, as, for example, protopine was increased up to 0.114% DW. by the eliciting but remained at trace level in cultures without elici-tor.
