摘要
湖北光周期敏感核不育水稻,在单核早期、单核晚期、二核及三核期的不育花药的细胞色素氧化酶(COD)、ATPase、过氧化物酶(POD)、过氧化氢酶(CAT)、SOD 在每小穗花药中的总活性普遍低于其可育花药,不育花药在发育的后期缺1—5条 COD、1条超氧物岐化酶(POD)和1—2条 Cu-Zn SOD 同工酶带。不育花药具较低的 ATP 含量和较高的 O(?)产生效率,且有 H_2O_2和丙二醛(MDA)的积累。对光敏雄性不育的发生与不育花药中异常的能量和活性氧代谢之内在联系进行了讨论。
The energy and activated oxygen metabolisms in male-sterile and fertile anth- ers of Hubei photoperiod-sensitive genic male-sterile rice(HPGMR)have been com- paratively studied.Among the male-sterile anthers,lower total activities of cyto- chrome oxidase(COD),ATPase,peroxidase(POD),catalase(CAT)and superoxide dismutase(SOD)in six anthers of a spikelet were seen,which were equivalent to about 2—64%,20—76%,26—60%,6—77%,29—55%,respectively,of those of their fertile counterparts during different development stages of pollens.The sterile anthers lacked 1—5,1,1—2 isozyme bands of COD,POD and SOD,respectively, and also displayed lower content of ATP,higher contents of H_2O_2 and malondiald ehyde(MDA)and higher efficiency of O_2^- which were equivalent to about 14— 77%,152—424%,153—238%,230—340%,respectively,of those of their correspon- ding fertile ones.It is concluded that the physiological features of male-sterile an- thers,viz.lower efficiency of oxidative phosphorylation,higher efficiency of O and H_2O_2 generation,weaker activity of scavenger system of activated oxygen and higher level of lipid peroxidation,are indicative of some relationship between ab- normal energy,activated oxygen metabolisms in male-sterile anthers and male-steri- lity in HPGMR.
基金
"863"和 Rockefeller 基金资助课题的一部分
关键词
活性氧
雄性不育
花药
水稻
Hubei photoperiod-sensitive genic male-sterile rice
Oxidative phosphorylation
Activated oxygen
Male-sterility
