摘要
目的 :观察神经营养素 3(neurotrophin 3,NT 3)基因转染大鼠失神经支配表情肌的情况及对其功能恢复的影响。方法 :离断大鼠面神经的下颌缘支和颊支后无张力外膜吻合制备面神经损伤模型 ,以直接注射法将脂质体DC Chol和重组质粒pcDNA3.1 (+) NT 3混匀后注入受损神经处的神经所支配的肌肉内。采用PT PCR技术检测转基因处肌肉的NT 3表达 ,并观察NT 3基因转染后大鼠触须活动情况。结果 :经脂质体DC Chol介导NT 3基因可有效地转染组织并得到表达 ,大鼠转基因实验侧的触须拂动比对照侧提前开始恢复。结论 :经脂质体介导的外源性NT
Objective:To observe the effect of neurotrophin 3(NT 3) gene transfer on the function recovery of facial muscle denervation in rats.Methods:The model of facial nerve lesion was established in rats by cutting the marginal mandibular branch and buccal branches of facial nerve.The recombinant plasmid pcDNA3.1(+) NT 3 and DC chol liposome admixture were injected into the muscles around the injured facial nerve.RT PCR was adopted to confirm whether NT 3 cDNA expressed after the injection,and the movement of the rats' palp was observed.Results:The muscles were infected by injecting the DC chol mediated NT 3 transfer and NT 3 gene was expressed.The movement of the rats' palp at the side injected NT 3 began to recover earlier than the control side.Conclusions:Transfer of NT 3 can promote the recovery of the function of the injured facial nerve by increasing local expression of exogenous NT-3.
出处
《蚌埠医学院学报》
CAS
2004年第2期104-105,F004,共3页
Journal of Bengbu Medical College
