摘要
目的 探讨心肌缺血预处理 (MIP)对未成熟心肌bcl- 2、bax、fas蛋白表达的影响。方法 采用兔Langen dorff模型 ,2 4只幼兔随机分为 3组 ,对照组 (C ,n =8) :仅灌注KH液 180min ,然后取标本 ;缺血 /再灌注组 (I/R ,n=8) :灌注 2 0min后 ,停灌 4 5min ,复灌 12 0min ;心肌缺血预处理组 (MIP ,n =8) ,灌注 2 0min ,反复 2次停灌 5min ,复灌 5min ,然后重复I/R组缺血 /再灌方法。以凋亡细胞原位标记、琼脂糖凝胶电泳检测DNA片段梯及bcl- 2、bax、fas蛋白表达作为检查指标。结果 琼脂糖凝胶电泳检测DNA片段梯 :MIP组与I/R组比较 ,光密度明显减弱 ;MIP组与I/R组比较 ,bcl- 2表达明显增多 ,bax、fas表达明显减少。结论 MIP通过影响bcl- 2、bax、fas基因蛋白的表达减少心肌细胞凋亡。
OBJECTIVE To investigate the effects of myocardial ischemic preconditioning (MIP) on immature myocardial cell apoptosis and expression of bcl-2,bax,fas protein .METHODS Isolated working rabbit heart model was used in this study, 24 rabbits (aged 14～21 days) were divided into 3 groups (n=8) . Control group was only perfused with Krebs-Henseleit (KH) 180 minutes. Ischemia/reperfusion(I/R) group was perfused 20 minutes ,and then kept under ischemia 45 minutes, followed by reperfustion 120 minutes. Myocardial ischemic preconditioning (MIP) group was perfused 20 minutes ,and then repeatly ischemia 5 minutes followed reperfusion 5 minutes 2 times,finnaly reperfused 120 minutes. Such indexes, including the TUNEL, nucleosomal ladder of DNA fragments, and the expression of bcl-2, bax,and fas gene were detected.RESULTS MIP group there was reduction in TUNEL cell apoptosis, ladder of DNA fragments,expression of bax and fas gene,and increase in expression of bcl-2 gene.CONCLUSION This study demonstrated that MIP decrease immature myocardial cell apoptosis by affecting the expression of bcl-2, bax,and fas gene .
出处
《中国体外循环杂志》
2004年第1期25-27,43,共4页
Chinese Journal of Extracorporeal Circulation
基金
国家自然科学基金 (30 2 0 0 2 79)
