摘要
目的 研究重组生长激素 (rGH ,思增 )对大鼠移植型肝癌模型肿瘤细胞增殖的影响 ,以了解rGH的使用对治疗肝癌是否有不利影响。方法 用腹腔移植型雄性种鼠的含肿瘤细胞的腹腔液接种于幼鼠的皮下 ,制成皮下种植实体瘤鼠。接种 7d后 ,将实体瘤切成 2mm× 2mm× 3mm大小瘤组织块 ,再将瘤组织块植于 82只成年雄性大鼠的肝上而成移植型肝癌模型。随机均分成实验组和对照组。实验组术后第 3天从尾静脉分别给予rGH 0 .5U / (kg·d)和生理盐水 ,连续 5d。手术后 8,10 ,12d3个时段处死。大鼠、肝脏、肿瘤组织称重 ,全自动图像分析仪检测肝细胞及肿瘤细胞数目密度、核分裂数、核分裂指数 ;术后第 8天处死的大鼠同时行3H TdR活体掺入计数。结果 除术后第 8天对照组大鼠体重较术前明显减轻外 (P <0 .0 5 ) ,两组其他各时段术前术后差异均无显著性 (P >0 .0 5 ) ;肝重、肝细胞及肿瘤细胞数目密度、肿瘤重、核分裂数、核分裂指数 ,同一时段两组间差异均无显著性 (P >0 .0 5 ) ;术后第 8天 3H TdR活体掺入计数 ,两组差异无显著性 (P >0 .0 5 ) ;肿瘤重量在同一组不同时段差异有极显著性 (P <0 .0 1)。结论 静脉注射rGH与大鼠移植肝癌细胞的增殖无明显关系。
Objective To study the effect of reconbinant growrh hormone(rGH) on the tumor cell proliferation in the transplantation liver tumor models in rats,to explore the advantages and disadvantages of rGH in the treatment of liver carcinoma.Methods The male weaning rats were used to vaccinate and transgenerate by intraperitoneal injection of abdominal fluid contarning tumor cells from the celiar transplantation tumor rats, the others weaning rats were used to be the solid tumor rats by subcutaneous vaccination with abdominal fluid of tumor cells.The solid tumor were divided into pieces (2 mm×2 mm×3 mm),which were inserted into the liver of the 82 adult male rats to establish the transplantation liver tumor models.and then the models were divided into experimental group and control group randomly.After 3 days,experimental group were given rGH (0.5U/ kg·d)and control group were given via caudal vein NS for 5 days.All the rats were killed in 8d,10d,12d,respectively.The rats, tumors and livers were weighted,the number of hepatocytes,carcinoma cells and mitotic index were measured,the number of 3 H-TdR incorporation in vivo was measured in 8 days after operation. Results There were no significant difference in all the results between the two groups,except the rat's weight of 8days after operation and the tumor weight in different phase.Conclusions The rGH has no significant effect on the proliferation of tumor cell in transplantation liver models in rats.
出处
《中国普通外科杂志》
CAS
CSCD
2004年第3期189-192,共4页
China Journal of General Surgery
关键词
生长激素/药理学
癌
肝细胞
疾病模型
动物
增殖
肿瘤细胞
培养的
肝肿瘤
实验性
CROWTH HORMONE/pharm
CARCINOMA HEPATOCELLULAR
DISEASE MODELS,ANIMAL
PROLIFERATION
LIVER VEOPLASMS,EXPERIMENTAL
TUMOR CELLS,CUITURED
