摘要
为研究污染物对氮循环中硝化过程的影响 ,利用摇瓶实验测定了 2 4种苯取代化合物对底泥氨氧化活性的抑制作用 .结果表明 ,单取代苯化合物对底泥氨氧化活性抑制作用强弱顺序为 :—OH >—NO2 >—NH2 >—Cl>—CH3 >—H ;取代基的位置对抑制作用有一定的影响 ,二甲苯对底泥氨氧化活性抑制作用强弱顺序为 :间 >邻 >对 ,苯二胺也表现为同样的趋势 ,但不明显 ;多取代甲基和氨基 ,随着取代基个数增加对底泥氨氧化抑制作用有增强趋势 .苯取代化合物对底泥氨氧化活性的抑制作用 (IC50 ,μmol·L-1)与取代基团电负性之和 ( ∑ E) 有较好的相关关系 :lgIC50 =14 72 - 0 91∑ E ,苯环上取代基团的∑ E越大 ,该化合物对底泥氨氧化作用的抑制越明显 .
The inhibition of 24 aromatics on ammonia oxidizing activity of nitrifying bacteria in sediment was measured. The effects of the kind, number and position of substituted groups on ammonia oxidizing activity of nitrifying bacteria were discussed. The inhibition of mono substituted benzenes on ammonia oxidizing activity of nitrifying bacteria were in order of —OH>—NO 2>—NH 2> —Cl> —CH 3>—H. The position of substituted groups of di substituted benzenes also affected the inhibition, and the inhibitions of dimethylbenzenes(xylene) were in order of meta > ortho > para . The increase in number of substituted group on benzene ring enhanced the inhibition of aromatics studied in this study on nitrifying bacteria. There was a linear relationship between inhibition (IC 50 ,μmol·L -1 of aromatics on ammonia oxidizing activity and total electronegativity (∑E) of aromatics: lgIC 50 =14 72- 0 91∑E .
出处
《环境科学》
EI
CAS
CSCD
北大核心
2004年第2期94-97,共4页
Environmental Science
基金
国家重点基础研究发展规划项目(G19990 45 711)
国家自然科学基金资助项目 (2 0 2 770 2 5)
教育部留学回国人员启动基金项目
关键词
苯取代化合物
底泥
氨氧化活性
抑制作用
硝化活性
aromatics
sediment
inhibition
ammonia oxidizing activity
nitrification activity
