摘要
目的 研究大鼠耳蜗螺旋神经节细胞中γ-氨基丁酸 (γ- aminobutyric acid,GABA) A受体α2 m RNA的表达并探讨其意义。方法 采用大鼠耳蜗石蜡切片 ,地高辛标记的 GABAAα2 c DNA探针 (5 4 9个碱基 ) ,抗地高辛的碱性磷酸酶检测系统 ,碱性磷酸酶显色剂 ,原位杂交检测大鼠耳蜗螺旋神经节细胞中 GABAAα2 m RNA受体的表达。结果 大鼠耳蜗中螺旋神经节细胞及其纤维均显示阳性杂交信号 ,表达 GABAAα2 受体 m RNA。骨螺旋板等其他结构不表达。作为阳性组织对照的小脑蒲肯野氏细胞和颗粒细胞的胞体及轴突表达 GABAAα2 受体。嗅上皮雌激素受体 c DNA探针阴性对照、无探针空白对照及无地高辛抗体对照的耳蜗螺旋神经节细胞及小脑组织均不表达GABAAα2 受体。结论 GABAAα2 受体位于听觉传导通路的第一级神经元——所有的 型螺旋神经节细胞及其纤维 ,强烈支持 GABA作为其递质 。
Objective To investigate the expression of γ-aminobutyric acid (GABA)A receptor α 2 subunit mRNA on rat cochlear spiral neurons and its' significance. Methods In the rat cochlear paraffin slides, the GABA Aα 2 receptor in spiral ganglion neuron was detected with in situ hybridization. Digoxigenin-GABA Aα 2 cDNA probe (549 base pair), anti-digoxigenin-AP(Fab fragments) and BM purple substrate(precipitating) were used. Results Positive signals(GABA Aα 2 mRNA) were seen in all cochlear spiral neurons and their nerves. GABA Aα 2 mRNA was not found in other structure such as bony spiral lamina. As a positive control, GABA Aα 2 mRNA was seen in Purkinje cells, granule cells and their axons in rat's cerebellum. GABA Aα 2 mRNA was not found in OMP cDNA probe (olfactory membrane estrogen receptor probe) negative control, non-probe negative control and non-anti-digoxingenin control in cochlear spiral neuron and cerebellum. Conclusion GABA Aα 2 receptor has been found in type Ⅰ spiral neurons and their nerves. It strongly indicates that GABA as a nerve transmitter plays an important role in inhibiting the excessive stimulation transmission of the inner hair cells.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2004年第2期182-184,187,共4页
Journal of Sichuan University(Medical Sciences)
关键词
螺旋神经节
Γ-氨基丁酸
受体
神经递质
原位杂交
Spiral ganglion neuron GABA Receptor Neurotransmitter in situ hybridization
