摘要
目的 扩增嗜肺军团菌 mip基因 ,导入载体 p UC18和 pc DNA3.1(+) ,构建重组质粒 p L pmip和pc DNA3.1- mip。方法 采用 PCR从嗜肺军团菌扩增得到 mip基因 ,导入载体 p UC18和 pc DNA3.1(+) ,构建重组质粒p L pmip和 pc DNA3.1- mip并转化大肠杆菌 ,用限制性酶切分析、PCR、序列分析进行鉴定。结果 扩增出 82 8bp的 mip基因 ;构建重组质粒 p L p Mip和 pc DNA3.1- mip。结论 成功扩增 82 8bp的嗜肺军团菌 Mip基因 ,构建重组质粒 p L p Mip和 pc DNA3.1- mip。
Objective To construct recombinant plasmid pLpmip and pcDNA3.1 mip of mip gene. Methods The mip gene was amplified by PCR from a template of Legionella pneumophila. The amplified DNA was ligated into pUC18 and pcDNA3.1(+) vector. The recombinant plasmid and pcDNA3.1 mip were identified by restriction analysis and PCR and further confirmed by sequence analysis. Results mip gene of 828bp was amplified. Recombinant plasmid pLpmip and pcDNA3.1 mip was constructed. Conclusion We amplified the mip gene of Legionella pneumophila, constructed recombinant plasmid pLpmip and pcDAN3.1 mip successfully.
出处
《西部医学》
2004年第1期1-2,共2页
Medical Journal of West China
基金
国家自然科学基金
四川大学青年科学基金项目(No:30 30 0 30 2 )
关键词
嗜肺军团菌
MIP
PCR
Legionella pneumophila
mip
PCR
Gene expression
