摘要
目的 :研究SKI 2 0 5 3R对胃癌细胞株SGC 790 1的作用及其机制。方法 :MTT法检测SKI 2 0 5 3R对SGC 790 1的增殖抑制作用 ,流式细胞术检测药物作用前后细胞周期的变化及增殖细胞核抗原(PCNA)的表达改变。结果 :SKI 2 0 5 3R可明显抑制SGC 790 1的增殖活性 ,改变SGC 790 1的细胞周期分布 ,降低PCNA的表达。结论 :SKI 2 0 5 3R对SGC 790 1有明显的杀伤和抑制增殖的作用 ,其机制可能与下调PCNA的表达有关。
Objective To study the effect of SKI-2053R on SGC-7901 cell line.Methods MTT assay was used to test the effects of SKI-2053R on SGC-7901 cells proliferation.Flow cytometry was employed to measure changes of the cell cycle and the expression of PCNA after the treatment of the drug.Results SKI-2053R inhibited proliferation of SGC-7901 cell line,changed the distribution of the cell cycle and decreased the expression of PCNA of the cancer cell.Conclusions SKI-2053R can significantly inhibit the proliferation of SGC-7901 cell line and the action mechanism may be correlate with the low expression of PCNA of the cancer cell.
出处
《肿瘤防治杂志》
2003年第11期1156-1158,共3页
China Journal of Cancer Prevention and Treatment
关键词
抗肿瘤药/药理学
胃肿瘤/药物疗法
肿瘤细胞
培养的
增殖细胞核抗原/分析
细胞周期
antineoplastic agents/pharmacology
stomach neoplasms/drug therapy
tumor cells,cultured
proliferating cell nuclear antigen/analysis
cell cycle
