摘要
目的 以肝细胞癌裸鼠动物模型为对象 ,研究鱼肝油酸钠经皮瘤内注射治疗肝癌的疗效及其机制。方法 肝癌模型裸鼠 2 8只 ,随机分成两组 ,分别给予鱼肝油酸钠及生理盐水局部浸润注射治疗。每隔 2天局部注射 1次 ,共 3次。治疗前后分别测肝、肾功能及白细胞计数。第一次注射后两组各随机选取 4只裸鼠 ,分别依次切取肿瘤组织进行病理组织学检查。完成实验 ,对所有动物行解剖病理检查 ,明确有无内脏及淋巴结转移情况。结果 第一次注射后 ,治疗组病理切片报告较对照组有不同程度的血管内血栓形成。实验结束时 ,治疗组肿瘤全部坏死脱落 ,与对照组肿瘤体积比较有显著性差异 (P <0 .0 0 1)。治疗前后两组肝、肾功能检查及白细胞计数未见统计学差异 (P >0 .0 5 )。结论 鱼肝油酸钠瘤内注射治疗具有显著效果 ,肿瘤血供障碍是一重要作用机制。并且该药无明显肝、肾毒性。
Objective To illustrate the antitumor effect and mechanism of human liver cancer xenografts in nude mice by means of percutaneous local injection of sodium morrhuate.Methods Twenty-eight five-week-old nude mice were planted subcutaneously with 7721-line fragments of human liver cancer tissue at the backs of their right axillas. The nude mice were divided into two groups randomly for sodium morrhuate and saline injection respectively,once every two days,3 times in total. Liver and kidney function tests as well as white blood cell counts(WBCs ) were determined before and after injections. In each group,4 nude mice tumors mass were resected at 5 minutes,10 minutes,24hours,and 48 hours after the first injection to observe thrombosis. Two weeks after the last injection,all nude mice were killed,and investigated by autopsy and histology for the information of organ and lymph node metastasis.Results There were more thrombi in the vessels around tumors in the treatment group than control group. All the tumors in treatment group were disappeared when the experiment was terminated. The sizes between two groups have a statistical significant difference (P<0.001).There were no evident changes of liver and kidney function as well as WBCs before and after treatment between the two groups (P>0.05).Conclusion Percutaneous local injection of sodium morrhuate has effective treatment of liver cancer,and without any obvious toxicity or side effects. Embolism of peri-tumor vessels obstruct tumor blood supply might be one of the positive factors.
出处
《中国医学影像技术》
CSCD
2004年第2期203-205,共3页
Chinese Journal of Medical Imaging Technology
关键词
鱼肝油酸钠
肝细胞癌
治疗
Sodium morrhuate
Hepatocellular carcinoma
Therapy
