摘要
采用CP(Colophony-Paraffin,CP,专利号:ZL98125709.7)切片和定向包埋技术,首次对黑腹果蝇(Drosophilamelanogaster)的头部进行了3μm的连续切片及制片,经常规染色后,在光学显微镜下对其视觉系统的显微结构进行了详细的观察研究.结果表明,黑腹果蝇的视觉系统主要由复眼、视叶和一些纤维通路组成.复眼由许多形态相同的小眼构成,每个小眼由外向内分别由半球形的角膜、倒锥形的晶体和柱形的视觉柱组成,视觉柱中有7个感光细胞,其轴突穿过复眼底部的视神经孔与视叶相连;视叶由外向内又依次分为神经节层、外髓、内髓板和内髓4个髓质区以及视网膜底部与神经节层之间的网膜后交叉、神经节层与外髓之间的外交叉和外髓与内髓之间的内交叉.黑腹果蝇视觉系统中主要包括角膜细胞、晶锥细胞、网膜细胞(光感细胞)、色素细胞、柱状神经元、无长突细胞和切向神经元7种类型的神经细胞,其胞体直径均小于3μm.
By colophony-paraffin(CP) directional embedding technique,made three-micron serial sections for the brain of Drosophila melanogaster for the first time . After routine staining, the microscopicstructure of the visual system was shown under a light microscope. The results show as follow: the visual system of Drosophila melanogaster mainly comprises the compound eyes, the optic lobes and many fiber tracts. Each compound eye contains many morphologic ally identical ommatidia. From distal to proximal, each ommatidium is composed of a half-spherical cornea, inverted pyramidal crystalline body and a columnar visual column with seven retinula cells. The axons of the retinula cells connected with the optic lobe by the foramen opticum of pars basilaris compound eye. The optic lobe consists of four major neuropil regions:the distalmost lamina, the medulla, the lobula plate and the most central lobula. Three horizontal chiasmata of fibers were observed in the connections between pars basilaris retina and lamina, the lamina and medulla, as well as medulla and lobula. The visual system of Drosophila melanogaster mainly comprises six categories of neurons: corneal cell, retinal cell (photoreceptor cell), pigment cell, columnar neuron, amacrine cell and tangential neuron. The diameters of those somata all less than 3 μm.
出处
《东北师大学报（自然科学版）》
CAS
CSCD
北大核心
2004年第1期71-77,共7页
Journal of Northeast Normal University(Natural Science Edition)
基金
国家自然科学基金资助项目(39940019)
科技部
教育部国家科技经费资助项目(JG-99-20)
关键词
黑腹果蝇
视觉系统
显微结构
Drosophila melanogaster
visual system
microstructure
