摘要
简要介绍了浮游植物种类和数量分析的8种方法的研究进展、应用现状,比较了各种方法的特点。基于浮游植物的外观形态特征而进行分析的方法除了传统的显微镜方法外,与计算机技术相结合的图像法使分析速度得以提高。基于浮游植物的色素组成而进行分析的有吸光光度法、荧光分光光度法和高压液相色谱法,可分至门类,同时定量,其中光度法可以直接测量海水,高压液相色谱法需萃取色素。流式细胞仪法的特点是可同时测量每个细胞的多个参数,运用神经网络分析可鉴别种类,应用时主要受限于仪器的操作和价格;化学发光流动注射法目前仅限于特定赤潮浮游植物的检测;分子探针法基于浮游植物的DNA特征,处于实验室研究阶段。
This paper, introduction of eight analytical methods for identification of phytoplanktons and determination of their abundences and (comparison) of their research progress, application actualities and characteristics were made. A new method from the traditional microscopic (method) (which) is based on morphology of phytoplankton, and image technology which is combined with computer technology, is increased the (analysis) (speed) sharply. Absorption spectra, spectral fluorescence and HPLC analysis, which are based on measurements of phytoplankton (pigment) (compositions), could assess phytoplankton class abundance. The difference is that optical method could directly use seawater while HPLC needs (extraction) of pigments. The chatacteristic of flow cytometric lies in its ability to detect several parameters at the same time and to identify the (phytoplankton) species by the radial basis function neural network analysis, but the complicated operations and high price of flow cytometry (limit) its application in situ . The chemiluminescence flow-injection method is used to detect specific red tide species. Molecular probe method is based on DNA sequences of phytoplanktons, which is at the stage of laboratory research.
出处
《海洋环境科学》
CAS
CSCD
北大核心
2004年第1期73-76,共4页
Marine Environmental Science
基金
国家863青年基金资助项目(2002AA639620)
国家重点基础研究发展规划项目(2001CB09703)
国家重点自然科学基金项目(40136020)
