摘要
3种海洋浮游硅藻,网纹三角藻(Triceratiumreticulum),骨条藻(Skeletonemasp.)和三角褐指藻(Phaeodacty lumtriconutum)培养在3种不同的光暗周期下,研究了光暗周期对硅藻胞外多糖生产的影响.结果表明,光暗周期对硅藻胞外多糖生产的影响具有种间特异性,且与细胞生长周期有关.网纹三角藻和三角褐指藻在长光照条件下(L/D:16/8h),胞外多糖含量较高,前者最大值出现在生长期前期,而后者则在生长期后期;骨条藻在短光照条件下(L/D:8/16h)胞外多糖含量较高,最大值出现在生长期后期.3种硅藻中,网纹三角藻的胞外多糖的产量最高,达103～104pg/cell,中肋骨条藻和三角褐指藻则较低.光暗周期可能影响了细胞内光吸收总量、细胞分裂模式及细胞内有机物质的合成,从而影响到胞外多糖的生产分泌.
Three marine planktonic diatom species, Triceratium reticulum, Skeletonema sp. and Phaeodactylum triconutum were cultured in modified f/2 medium, and the influence of light-dark cycle on the production of extracellular polysaccharide was investigated. The results showed that the influences of light-dark cycle on production of extracellular polysaccharide were species specific, and dependent on the growth phase. The extracellular polysaccharide content of T. reticulum and P. triconutum was higher under long light cycle (L/D, 16/8h), however the highest content was found at the exponential phase in T. reticulumn and at the late growth phase in P.triconutum. The content of extracellular polysaccharide in Skeletonema sp. was higher under longer dark cycle (L/D, 8/16h) and peaked at the late growth phase. Among the three species, the production of extracellular polysaccharide (pg·cell^(-1)) was the highest in T. reticulumn, up to 10~3～10~4 pg·cell^(-1), while the production of P.triconutum and Skeletonema sp. was very low. Light-dark cycle might affect the total amount of light absorption, cell division pattern and synthesis of organic materials, which inversely influenced the extracellular polysaccharide production of algal cells.
出处
《厦门大学学报（自然科学版）》
CAS
CSCD
北大核心
2004年第2期244-248,共5页
Journal of Xiamen University：Natural Science
基金
国家自然科学基金(39900020)
教育部青年骨干教师项目资助
