摘要
本文对红托竹荪(Dictyophora rubrovalvata)多糖的提取、纯化和单糖的组成成分进行了研究。红托竹荪子实体经热水提取、乙醇沉淀得多糖(Dr)粗品,多糖粗品经脱蛋白,乙醇分级沉淀、Sephadex G-15葡聚糖凝胶柱层析纯化得Dr-1,Dr-2,Dr-3三个级分,应用薄层层析技术,确定该三个级分均由半乳糖、葡萄糖、甘露糖和木糖组成。
In this paper, the crude polysaccharides from the fruitbody of mushroom Dictyophora rubrovolota were obtainedby extracting with hot water and precipitating with alcohol. The free proteins were removed from crude polysaccharides, andthen the polysaccharides were isolated and purified by Sephadex G-15 gel column chromatography. Three pure polysaccharides(Dr-1, Dr-2 and Dr-3) were obtained by precipitating with different concentration alcohol. By using the TLC method, we provedthat the three fractions were all composed of galactose (Gal), glucose (Glu), mannose (Man) and xylose (Xyl).
出处
《食品科学》
EI
CAS
CSCD
北大核心
2004年第3期43-45,共3页
Food Science
基金
贵州省优秀科教人才基金[黔科教办(2001)3号]
关键词
红托竹荪
多糖
提取
纯化
分离
薄板层析
化学结构
食用菌
Dictyophora rubrovolota
polysaccharides
isolation
purification
TLC (Thin-layer-chromafography)
