摘要
目的 探讨noggin基因对成年海马BrdU标记细胞的调控。方法 原位杂交与RT PCR检测noggin基因在海马的表达 ,免疫组化检测BrdU标记细胞数 ,反义技术阻断内源性noggin基因表达。结果 侧脑室注射反义寡核苷酸后 ,成年大鼠海马内nogginmRNA阳性细胞数明显降低 ,而BMP4mRNA阳性细胞数无明显变化 ,同时大鼠海马齿回及齿回颗粒下区的BrdU阳性细胞数均较正常对照组明显降低 ,正义寡核苷酸无此效应。结论 内源性noggin可促进成年内海马神经前体细胞的增殖。
Objective To investigate the effect of noggin on BrdU labeled cells in the adult rat hippocampus. Methods The expressions of noggin and bone morphogenetic protein 4 (BMP4) in rat hippocampus were detected using in situ hybridization histochemistry (ISHH) and reverse transcription polymerase chain reaction (RT PCR). By using antisense technique combined with bromodeoxyuridine!(BrdU) labeling, the effect of noggin on hippocampal neurogenesis in adult rats was explored. Results The number of noggin mRNA positive cells in the adult rat hippocampus decreased significantly after treatment with antisense noggin but no change was found in the number of BMP4 mRNA positive cells. In addition, the number of BrdU labeled cells decreased significantly in the adult rat hippocampus after treatment with antisense noggin, but the sense noggin had no such effect. Conclusion Noggin can promote proliferation of neural precursor cells in adult rat hippocampus.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2003年第15期1311-1314,共4页
Journal of Third Military Medical University
基金
全军"十五"科研基金资助课题 ( 0 1Q10 9)
