摘要
目的:以C57BL/6小鼠为动物模型,探讨中药黄芪、女贞子、人参混合煎剂促进C57BL/6小鼠毛发生长的可能机制。方法:中药组小鼠给予中药混合煎剂口饲,对照组给予等量生理盐水,观察小鼠背部皮肤颜色变化和毛发生长情况。同时在光镜下观察小鼠毛囊组织学变化及毛囊内细胞的凋亡的状况。结果:中药组小鼠饲饮中药煎剂后背部皮肤颜色由粉红色变为黑色比对照组提前了1d,背部皮肤颜色由黑色变为灰黑色比对照组延长了1.5d。组织学上,拔毛后第18天对照组小鼠毛囊处于退行中晚期,而中药组仍为生长Ⅵ期和退行早期毛囊。中药组小鼠毛囊内凋亡细胞数量比对照组减少(P<0.001)。结论:黄芪、女贞子、人参混合煎剂促毛发生长作用可能与抑制退行期毛囊内细胞凋亡,诱导和延长C57BL小鼠毛发的生长期有关。
Objective:To investigate the possible mechanisms of effects of Chinese herbal medicine on promoted murine hair follicles growth in vivo.Methods:C57BL/6mice as animal model,the water fraction of herbal medicines was adminis trated since day-7post depilation(p.d.).On day10and18p.d.,the skin on the depilated area was harvested for histology,and the detection of pop to sis.Results:On day18p.d.,hair follicles in herbs-treated mice were still in an agenⅥand early cata-gen,whereas hair follicles in control mice were in mid-catagen and late catagen histologic ex am ination.On day18p.d.,herbal group showed little cells stained with TUNEL for fragmented DNA,whereas control group showed massive follicular apoptosis.Conclusions:These data sup port that Chi nese herbal medicine can promote murine hair growth,possibly via sup-pressing follicular apoptosis of normal cata gen folli cles,prolonging anagen and delaying catagen development.
出处
《临床皮肤科杂志》
CAS
CSCD
北大核心
2003年第6期321-322,共2页
Journal of Clinical Dermatology
基金
国家自然科学基金(项目编号30170860)
江苏省中医药管理局基金(项目编号9975)
关键词
黄芪
女贞子
人参
毛发生长
C57BL/6小鼠
Astragalus membranaceus
Ligustrum lucidum
Panax ginseng
hair growth
C57BL/6mice
