摘要
目的 评价腺病毒介导的人骨形态发生蛋白 2 (Adv hBMP 2 )基因转染的组织工程化人工骨对大动物长骨干骨缺损的修复效果。方法 建立羊胫骨干骨缺损模型 (2 .6cm) ,单侧 (右 )植入体外构建的组织工程化骨 ,2 6只羊分为 5组 :(1)Adv hBMP 2转染的骨髓间充质干细胞 (BMSC)加煅烧骨 (CB)组 (9侧 ) ;(2 )腺病毒介导的 β 半乳糖苷酶 (Adv βgal)基因转染的BMSC加CB组 (6侧 ) ;(3)未转染的BMSC加CB组 (6侧 ) ;(4)单纯CB组 (3侧 ) ;(5 )未治疗组 (2侧 )。分期行X线、计量组织学检查和生物力学测定。结果 X线 :4～ 8周 ,Adv hBMP 2转染组的羊胫骨干骨缺损内有明显骨痂形成 ;2 6周 ,(1)、(2 )、(3)、(4)和 (5 )组的完全愈合率 ,分别为 5 / 8、1/ 5、0 / 5、0 / 2、0 / 1。计量组织学示 ,与其他组相比 ,1组的新生骨量最多 ,并有皮质骨形成 ;第 1组移植骨的压缩载荷和弹性模量明显大于其他各组。结论 hBMP 2基因修饰的组织工程化人工骨可以修复大动物 (羊 )负重骨 (胫骨 )的节段性缺损。
Objective To evaluate the effectiveness of the tissue-engineered bone substitute loaded with adenovirus mediated human bone morphogenetic protein-2 gene (Adv-hBMP-2)transfected bone marrow derived mesenchymal stem cells(BMSC)in the repair of diaphyseal segmental bone defect of large animal. Methods The right tibial bone defects (2.6 cm) model of 26 goats were established and divided into 5 groups: I.Adv-hBMP-2 transfected BMSC/calcined bone(CB) group ( n =9); II.adenovirus-β-galactosidase (Adv-βgal) gene transfected BMSC/CB group ( n =6); III.untransfected BMSC/CB group ( n =6); IV.single CB group ( n =3); VI.untreated group ( n =2). The above tissue-engineered bone substitutes were implanted in the bone defects respectively except group VI. Roentgenography, histomorphometrical analysis and biomechanical measurement were studied at various times. Results X-ray: at 4～8 th weeks after implantation, more bony callus was found in the bone defects of group I. The complete healing rates of group I, II, III, IV, and V were 5/8, 1/5, 0/5, 0/2, 0/1 respectively at 26 th week after implantation. Histomorphometrical analysis showed much more new bony callus including cortical bone formed in group I than those of other groups. The compression strength of the implanted bone substitute of group I is significantly higher than those of group II and III. Conclusion The tissue-engineered bone substitute loaded with human BMP-2 gene transfected BMSC can repair diaphyseal segmental bone defect of large animal (goat).
出处
《中华医学杂志》
CAS
CSCD
北大核心
2003年第15期1345-1349,共5页
National Medical Journal of China
基金
上海市重点科技发展项目 (0 1JC14 0 2 8)
上海市科委国际合作项目 (0 14 3 0 70 2 1)
