摘要
目的 建立测定人血浆中头孢泊肟的反相高效液相色谱检测法。方法 血浆样品用高氯酸溶液沉淀后上清液直接进样。色谱柱为C18柱 ,15 0mm× 4 .6mm ,5 μm ,流动相为 1.5 0mmol·L-1高氯酸溶液 乙腈 (85∶15 ) ,流速为 1.2 0mL·min-1,紫外检测波长 :2 5 4nm。外标法峰面积定量。结果 测定方法在 0 .10～ 6 .0 0mg·L-1内具良好的线性关系 ,回收率为 98.0 %～ 110 .0 % ,日内、日间RSD小于 13% ,最低检测浓度为 0 .10mg·L-1。结论 本测定方法具灵敏、准确、快速的优点 ,适用于头孢泊肟酯片剂的药代动力学和生物利用度研究。
OBJECTIVE To develop a simple, sensitive and rapid RP-HPLC method for the determination of cefpodoxime in human plasma. METHOD The plasma samples were extracted with 1.25 mol×L -1 perchloric acid. The analysis involved a 150mm×4.6mm ID column packed with C 18( 5μm ). The mobile phase consisted of 1.50 mmol·L -1 perchloride acid solution-acetonitrile( 85∶15 ) with a flow rate of 1.20 mL·min -1. The UV detector were set at 254nm.RESULTS A good linearity was obtained in the rang of 0.10 ～ 6.00 mg·L -1( r=0.9999 ). The recovery was between 98.0% ～ 110.0%. The relative standard deviation of within-day and between-day were less than 13% ( n=6 ). The minimal detectable concentration in plasma was 0.10 mg·L -1. CONCLUSION The established method is sensitive, accurate and simple for the determination of cefpodoxime levels in human plasma. It is suitable for the pharmacokinetics and bioavailability study of cefpodoxime proxetil.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2004年第1期51-53,共3页
Chinese Journal of Modern Applied Pharmacy
