摘要
目的:研究舒脉胶囊对大鼠心肌缺血再灌注损伤的影响。方法:制备大鼠心肌缺血再灌注损伤模型,用化学比色法测定血清肌酸激酶(CK)、乳酸脱氢酶(LDH)含量及心肌组织匀浆丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性,用放免法测定血浆内皮素(ET)及降钙素基因相关肽(CGRP)含量。结果:大鼠心肌缺血10min,再灌注30min后,血清CK水平明显升高,血浆ET含量增加,心肌组织匀浆SOD活性下降。舒脉胶囊可显著降低缺血再灌注大鼠血中CK、ET水平及心肌组织MDA含量,显著升高血中CGRP水平及心肌组织SOD活性。结论:舒脉胶囊对大鼠心肌缺血再灌注损伤具有保护作用,其作用机制可能与扩张血管、提高氧自由基清除酶活性、抑制脂质过氧化反应有关。
Abjective: To investigate the protective effect of Shumai capsule on myocardial ischemia reperfusion injury in rats. Methods: Rats were prepared to produce a model of myocardial ischemia reperfusion injury by coronary artery ligation for 10 min followed by reperfusion 30 min. Malondialdelyde (MDA) content and superoxide dismutase (SOD) activity in myocardium, serum creatine kinase (CK) and lactate dehydrogenase (LDH) were assayed by spectroscopy respectively. Plasma endothelin (ET) and calcitonin gene-related peptide (CGRP) were assayed by radio-immunoassay. Results: CK and ET level increased significantly while SOD level decreased significantly in ischemia-reperfusion rats compared with those in the sham group. Shumai capsule can decrease CK, ET, MDA content and increase CGRP, SOD level significantly. Conclusion: Shumai capsule has protective effect on myocardial ischemia reperfusion injury in rats. The protective effect may be that it can dilate vessels, increase the activity of clearence enzyme of free radical, and inhibit lipid peroxidation.
出处
《山东大学学报（医学版）》
CAS
2004年第1期12-15,共4页
Journal of Shandong University:Health Sciences
基金
山东省科技厅资助课题(991205706)
关键词
舒脉胶囊
缺血再灌注
抗氧化药
自由基
大鼠
近交系
Shumai capsule
Ischemia-reperfusion
Antioxidant
Free radicals
Rats, inbred strain
