摘要
在含Cd、Cu浓度100、200μMol的培养基中进行驯化培育,获5株抗Cd细胞株及3株抗Cu细胞株。驯化株细胞相对增长率显著高于亲株,在Cd、Cu浓度波动较大的条件下,驯化株细胞增长率仍较稳定;驯化株对Cd、Cu的蓄积性因细胞种类不同而异,或具有降低对Cd、Cu的吸收,使抗性增加的机制,或对Cd、Cu有更强的吸收特性,但浓缩系数相对较稳定,从而抵御环境中Cd、Cu的波动,驯化株对营养元素的吸收呈现高于亲株或低于(近于)亲株两种类型,推断具有通过新陈代谢调节的适应机制;终止驯化的回复株一定程度保留了驯化获得性,但有向亲株回复的趋势,推断所获抗性非基因控制类型。
Five Cd-resistant and three Cu-resistant plant cell lines are obtained by cultivation in media containing 100 and 200 μmol Cd and Cu.Their relative multiplying rates are significantly higher than those of their parents and remain stable even with an obvious fluctuation of the concentrations of Cd and Cu. The accumulation of Cd and Cu by the cultured lines varies with the type of cells: some can reduce the absorption of Cd and Cu to raise their resistance, and others can absorb more Cd and Cu, but have a relatively stable accumulation to withstand the fluctuation of Cd and Cu in the environment. The nutrient absorption of cultured lines is stronger or weaker than that of their parents, suggesting that they are adapted to the environment by metabolism regulation. To some extents, the lines retain their characteristics obtained from cultivation, but still have a trend of reversing to their parents characterislics. It's thought that the tolerance is non geno-controlled.
出处
《应用生态学报》
CAS
CSCD
1992年第3期273-279,共7页
Chinese Journal of Applied Ecology
基金
国家自然科学基金
关键词
重金属
植物抗性
细胞培养
Heavy metal, Plant resistance, Cell culture.
