摘要
背景:数十年来国内外众多学者对哮喘的发病机制和治疗进行了大量的研究,但少有对哮喘脑超微结构影响的报道。目的:探讨慢性哮喘动物模型海马超微结构变化及其在低氧干预后的改变。设计:完全随机对照实验研究。地点与材料:本研究的地点为第三军医大学西南医院儿科。材料为第三军医大学实验动物中心提供的青年豚鼠16只,雌雄各半,平均体重(200±25)g。干预:复制慢性哮喘动物模型,分为低压低氧(低压舱)治疗组、常压低氧吸入治疗组及不治组,并设正常对照组。主要观察指标:电镜观察海马超微结构变化。结果:电镜下慢性哮喘豚鼠海马神经元粗面内质网扩张,线粒体脊断裂、模糊,突触小泡减少、突触后膜稍肿胀;星状、小胶质细胞增生、胞浆肿胀、空泡形成;血管周围间隙增宽、基底膜肿胀、模糊、管腔狭窄。低氧干预后海马神经元核仁明显,细胞器丰富,粗面内网轻度扩张,突触小泡较丰富,突触后膜较致密,突触间隙略显增宽,胶质细胞及血管未见明显病变。结论:反复以作的性是哮喘可因缺氧致海马超微结构发生变化,经低压氧及常压低氧治疗后因哮喘所致的海马超微结构损伤明显好转。
BACKGROUND:In the past decades, scholars in the world have large numbers of studies on the pathogenesis and treatment of asthma;however,the reports about the changes in cerebral ultrastructure of asthma were rare.OBJECTIVE:To observe the ultrastructural character of hippocampus in guinea pig of chronic asthmatic model and changes after hypobaric oxygen treatment. DESIGN:Completely randomized case-control study was adopted. SETTING and MATERIALS:The study was completed in the Department of Pediatrics,Southwest Hospital of Third Military Medical University.Sixteen adult guinea pigs,with the average weight of(200±25) g,were provided by Experimental Animal Center of Third Military Medical University without limitation of sex.INTERVENTION:Chronic asthmatic models were successfully established in twelve guinea pigs that were randomly divided into three groups receiving hypobaric hypoxia treatment,normal pressure and hypoxia treatment and no treatment respectively,and other four guinea pigs were selected as normal controls.MAIN OUTCOME MEASURE:The ultrastructural changes in hippocampus were observed under electron microscopeRESULTS:The pathological changes were observed under electron microscope in chronic asthmatic guinea pigs,including dilated neuronal rough endoplasmic reticulum,broken and indistinct mitochondria ridge,and reduced synaptic vesicles with slightly swelling post-synaptic membrane. Star-like and small glia cells hyperplasia and swelling cytoplasm were observed with the formation of vacuoles.Blood vessels showed dilated clearance around,and swelling,distinct basal membrane with narrow cavity.After hypobaric oxygen treatment,nuclear of neurons appeared distinct,with plenty of organelles,and rough endoplasmic reticulum showed slightly dilated, with abundant synaptic vesicles,dense postsynaptic membrane and widened synaptic intervals.No obviously abnormal changes were found in glia cells and vessels. CONCLUSION:Recurred chronic asthmatic causes the changes of hippocampal microstructure due to hypoxia, which can be improved by hypobaric and hypoxia treatment,as well as normal pressure and hypoxia treatment.
出处
《中国临床康复》
CSCD
2004年第7期1370-1371,F003,共3页
Chinese Journal of Clinical Rehabilitation
