摘要
RNA干扰 ( RNA interference,RNAi)是正常生物体内抑制特定基因表达的一种现象 ,它是指当细胞中导入与内源性 m RNA编码区同源的双链 RNA( double stranded RNA,ds RNA )时 ,该 m RNA发生降解而导致基因表达沉默的现象 ,这种现象发生在转录后水平 ,又称为转录后基因沉默 ( post-transcriptional genesilencing,PTGS)。外源 ds RNA进入细胞后产生的小分子干扰 RNA( small interfering RNA,si RNA)的反义链和多种核酸酶形成了沉默复合物 ( RNA-induced silencing com plex,RISC) ,RISC具有结合和切割 m RNA的作用而介导RNA干扰的过程。 RNAi具有特异性和高效性。这种技术已经成为研究基因功能的重要工具 ,并将在病毒病。
A new phenomenon-RNA interference(RNAi) has been observed in many organisms recently, including eukaryote and prokaryote. When Double-stranded RNA(dsRNA) is introduced into cell, it would be decomposed to small interfering RNA(siRNA), and RNA-induced silencing complex(RISC) is formed out of siRNA and several enzymes. RISC can combine with mRNA and incise it, then the mRNA is disintegrated and the expression of gene is inhibited. The phenomenon is also called the sequence-specific post-transcriptional gene silencing(PTGS), and the process is charactered by high efficiency and peculiarity. RNAi has played an important role in the research of genic functions, and may yield RNA-based drugs to treat diseases,such as virus and tumor and genetc diseases.
出处
《动物医学进展》
CSCD
2004年第2期14-16,共3页
Progress In Veterinary Medicine
