摘要
本实验采用低能量氦氖激光连续辐照不产核黄素的棉病囊霉酵母菌,在较高功率密度处获得突变菌落,通过薄层层析,高压液相色谱及荧光光谱分析方法,证明突变菌株产生新的代谢产物——核黄素。经过多次传代,进一步证实此诱变所得的新性状是可以稳定遗传的。
50mW He-Ne laser was used to irradiate Ashbya gossypii, which doesn't produceriboflavin, and mutants were obtained at 20 mw. Results of High Perfermence Thin LayerChromatography, Reverse Phase-HPLC and fluorenscent analysis show that one of the new metablicproducts is riboflavin. The mutantsstill produce riboflavin after generations of culture, indicating thatthe new radiation induced property is stably inheritable.
出处
《应用激光》
CSCD
北大核心
1992年第6期265-268,共4页
Applied Laser
关键词
棉病囊霉
激光诱变
核黄素
Ashbya gossypii
laser-induced mutation
riboflavin
