摘要
目的 评价羟基喜树碱对人肝癌BEL-7402细胞的细胞毒性。方法 将羟基喜树碱以不同终浓度作用于人肝癌BEL-7402细胞。用MTT比色法观察其细胞毒性,荧光显微镜、透射电镜检测凋亡细胞的形态改变并记数凋亡指数,流式细胞仪定量分析细胞凋亡率。结果 羟基喜树碱以剂量依赖的方式抑制BEL-7402细胞的生长;荧光显微镜和透射电镜观察到细胞皱缩、核质浓缩、核碎裂、细胞起泡以及凋亡小体形成等凋亡特征性的形态学改变;流式细胞仪直方图上可见亚二倍体峰;以浓度为12.5、25、50、100、200μg/ml的羟基喜树碱处理48 h后,细胞凋亡率分别为(14.68±2.17)％、(21.24±3.76)％、 (31.54±4.58)％、(40.21±5.26)％和(48.35±6.70)％,与对照组(2.09±0.42)％比较差异均有极显著性意义(P<0.01)。结论 羟基喜树碱既能抑制人肝癌BEL-7402细胞增殖,又能诱导其凋亡,显示出较强的细胞毒性。
Objective To evaluate the cytotoxicjty of hydroxyl camptothecinum (HCPT) on human hepatocellular carcinoma cell line BEL-7402 by means of growth inhibition and apoptosis induction in vitro. Methods The hepatocellular carcinoma cell line BEL-7402 was treated with HCPT at various concentrations. Growth suppression was evaluated by MTT method; apoptosis-related alterations in morphology were ascertained under cytochemical staining (Hoechst 33258), transmission electron microscopy (TEM). Apoptotic index was counted under fluorescence microscopy. Flow cytometry (FCM) was used to investigate the apoptotic rate. Results Hydroxyl camptothecinum could inhibit the growth of hepatoma cells in a dose-dependent manner. Under fluorescent staining and transmission electron microscopy, the cells treated with HCPT exhibited characteristics of apoptosis including cell membrane shrinkage, cytoplasm blebbing, condensation and fragmentation of nuclear chromatin and formation of apoptotic bodies. Flow cytometry a-nalysis revealed hypodiploid peaks on histogram, and the apoptotic rates at concentrations of 12. 5, 25, 50, 100 and 200 μg/ml hydroxyl camptothecinum were (14. 68 ±2. 17) %, (21.24±3.76) %, (31.54±4.58) %, (40. 21±5. 26) % and (48. 35±6. 70) % respectively, which were all significantly higher than those of control group (2. 09±0. 42) %. Conclusion HCPT not only inhibited proliferation but also induced apoptosis of human hepatocellular carcinoma cell line BEL-7402, demonstrating strong cytotoxicity.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2004年第1期71-74,共4页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
