摘要
目的:研究在双特异性抗体的介导下CD3AK细胞(CD3单克隆抗体激活的杀伤细胞)对人小细胞肺癌细胞株LTEP-sml细胞毒作用。方法:用51Cr-Na2CrO4释放试验检测单抗(2D6、UCHT1)和双特异性抗体(WST-HT)与CD3AK共同对人小细胞肺癌细胞株的杀伤活性。结果:双特异性抗体外能明显增强CD3AK细胞对人小细胞肺癌的杀伤活性。结论:与单纯CD3AK相比加入双特异性抗体后的CD3AK细胞的细胞毒活性显著增强。
Objective:Studied the cytotoxicity of CD3AK cells that imposed on small cell lung cancer with bispecif ic monocloned antibody. Methods: The cytotoxic activities of CD3 AK cells with monocloned antibody and bispecific monocloned antibody were determined by51Cr-Na2CrO4 release assay. Results: Bispecif ic antibody could significantly increase the killer activity of CD3AK cells out of the organism. Conclusion: compared with monocloned antibodies cytotoxicity of CD3AK cells was increased significantly after introduced bispecific monocloned antibody.
出处
《中国民康医学》
2004年第2期65-67,共3页
Medical Journal of Chinese People’s Health
关键词
双特异性抗体
CD3AK
体外杀伤实验
细胞毒作用
小细胞肺癌
bispecific monocloned antibody
anti-CD3 antibody induced activated killer cells
Na2 CrO4
small-cells lung cancer cell line(LTEP-sml)
cytotoxicity
