摘要
目的 :两株登革 2型病毒 (DengueType 2virus ,DV2 )初次和再次感染BALB C小鼠建立动物感染模型 ,对其产生特异性抗体进行动态观察 ,探讨感染DV2 NGC株和DV2 临床分离株 (B株 )引起的体液免疫应答的差异。方法 :两株DV2 毒株模拟自然感染途径 ,经皮下多点注射建立BALB C小鼠感染动物模型 ;采用间接ELISA法检测感染动物血浆中抗DV2 特异性IgM类抗体、IgG类抗体 ,并同时分离病毒观察病毒血症期的变化。结果 :不同DV2 毒株初次、再次感染BALB C小鼠后诱导特异性Ig产生的类别存在差异 ,且DV2 B株再次感染动物后表现为病毒血症期相对延长。结论 :两株DV2 诱导特异性抗体产生的动态与病毒血症期不同。
Objective:BALB/C mice model infected with different Dengue Type 2 virus (DV 2) strains was evaluated and dynamic patterns of humoral immune responses caused by the various viral strains were investigated.Methods:The BALB/C mice were multisubcutaneouly injected by different DV 2 strains. The dynamic changes of the anti-DV 2 IgM/IgG antibody were observed in the infected BALB/C mice by the indrect ELISA and the viremia was observed.Results:The productions of specific antibodies in the infected BALB/C mice after primary and secondary injection with different DV 2 strains showed some difference. Howerer, BALB/C mice infected by the DV 2 B strain from a DHF patient showed the prolonged viremia.Conclusion:The dynamic curve of specific antibody and the viremia induced by various DV 2 strain (DV 2 NGC strain.V.DV 2 B strain) were different in some existence.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2004年第2期75-78,共4页
Chinese Journal of Immunology
基金
国家自然科学基金资助 (资助号 :3 9960 0 0 1)
