摘要
目的 :观察探讨大白鼠脊髓损伤后细胞凋亡及调控基因Bcl -2、Bax的表达及意义。方法 :2 8只SD大鼠随机分为 7组 ,Allen′s法致伤脊髓 ,于术后 4、 8、 2 4、 48、 72、 168h采集脊髓标本 ,1组作为对照组 ,分别行HE染色、TUNEL染色和免疫组化技术检测Bcl -2、Bax的表达。结果 :TUNEL染色显示有神经元和胶质细胞凋亡。Bcl-2在术后 4h开始出现阳性表达 ,2 4h达高峰。Bax术后 4h出现阳性表达 ,8h时达高峰。结论 :脊髓损伤后存在神经元和胶质细胞的凋亡 ,Bcl-2、Bax基因对调控细胞凋亡可能具有重要作用。
Objective:To observe the expression of Bcl-2, Bax and the relationship with apoptosis in spinal cord injury. Methods:Twenty-eight SD rats were used in this experiment. Spinal cord impact was performed using Allen′s method. At the follow times (4, 8, 24, 48, 72, or 168hr), the spinal cord was taken. Then the observation of the pathological changes and apoptosis on the HE staining and TUNEL staining sections were performed.Immunohistochemistry methods were used to study the expression of Bcl-2, Bax gene. Results: TUNEL-positive cells were maximal at 8 hr after injury. There was a weak positive expression of Bcl-2 protein at 4hr after injury. By 24hr after injury, the positive expression of Bcl-2 was maximal. The positive expression of Bax was maximal at 8hr after injury. Conclusion: Apoptosis phenomenon presents in rat spinal cord injury. Bcl-2 protein and Bax gene may be the important factors influencing apoptosis and pathological processes after spinal cord injury.
出处
《中国矫形外科杂志》
CAS
CSCD
2004年第3期228-229,共2页
Orthopedic Journal of China
